Details

scope
Mouse cortex; modeling of GEVI 2P voltage imaging (JEDI-2P), with empirical 1P vs 2P measurements
claim_text
Two-photon voltage imaging in mouse cortex is shot-noise- and photodamage-limited: for JEDI-2P with SNR 10, 1 kHz bandwidth, 200 mW laser power, and >300 µm depth, an 80-MHz 2P source can record from at most ~12 neurons simultaneously.
section_id
section_16
source_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json
effect_size
~12 simultaneously recordable neurons (>300 µm depth, 200 mW, 80 MHz, SNR 10, 1 kHz)
review_repo
ComputationalReviewRecurrence
section_ref
wiki_page:computationalreviewrecurrence-16-synthesis
source_kind
review_finding
source_path
evidence/section_16_evidence_package.json
study_system
Mouse cortex; modeling of GEVI 2P voltage imaging (JEDI-2P), with empirical 1P vs 2P measurements
section_title
16. Synthesis — which computational claims the mouse-cortex E→E empirical record actually supports, where the bottleneck observations are, and what an inhibition-free, single-species, basic-research analytic framing misses
evidence_summary
Quantitative resolution-limit calculation for 2P voltage imaging — directly addresses the cluster_14 topic that voltage imaging cannot deliver the simultaneous, large-population subthreshold readout some E→E claims would require.
review_bundle_ref
analysis_bundle:ab-d9c479db9be9
replication_status
single-study
review_package_ref
analysis_bundle:ab-d9c479db9be9
source_artifact_ref
wiki_page:computationalreviewrecurrence-16-synthesis
origin_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json
commit_sha
79ce062d54a924ce05953ec90aa9d26044d2b48f
created_by
persona-jerome-lecoq-gbo-neuroscience
repository_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence
Raw fields (5)
raw_fields
{
  "n": 12,
  "doi": "10.1117/1.nph.11.3.035007",
  "claim": "Two-photon voltage imaging in mouse cortex is shot-noise- and photodamage-limited: for JEDI-2P with SNR 10, 1 kHz bandwidth, 200 mW laser power, and >300 µm depth, an 80-MHz 2P source can record from at most ~12 neurons simultaneously.",
  "cite_key": "PhilBrooks2024",
  "evidence": "Quantitative resolution-limit calculation for 2P voltage imaging — directly addresses the cluster_14 topic that voltage imaging cannot deliver the simultaneous, large-population subthreshold readout some E→E claims would require.",
  "effect_size": "~12 simultaneously recordable neurons (>300 µm depth, 200 mW, 80 MHz, SNR 10, 1 kHz)",
  "text_access": "abstract_only",
  "study_system": "Mouse cortex; modeling of GEVI 2P voltage imaging (JEDI-2P), with empirical 1P vs 2P measurements",
  "argument_role": "supporting",
  "replication_status": "single-study",
  "claim_source_sentence": "For voltage imaging with JEDI-2P in the mouse cortex with a target SNR of 10 (spike height to baseline shot noise), a measurement bandwidth of 1 kHz, a thermally limited laser power of 200 mW, and an imaging depth of > 300    μ m  , 2P voltage imaging using an 80-MHz source can record from no more than ∼ 12  neurons simultaneously.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "2P voltage imaging using an 80-MHz source can record from no more than ∼ 12  neurons simultaneously."
}
source_refs
[
  "paper:paper-ed927db80f97"
]
source_span
For voltage imaging with JEDI-2P in the mouse cortex with a target SNR of 10 (spike height to baseline shot noise), a measurement bandwidth of 1 kHz, a thermally limited laser power of 200 mW, and an imaging depth of > 300 μ m , 2P voltage imaging using an 80-MHz source can record from no more than ∼ 12 neurons simultaneously.
evidence_refs
[
  {
    "ref": "paper:paper-ed927db80f97"
  }
]
source_policy
{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}

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