Single-cell and multi-omics atlas of 568,654 transcriptomes plus 200,061 nuclei…

Single-cell and multi-omics atlas of 568,654 transcriptomes plus 200,061 nuclei sampled densely across mouse V1 development (E11.5 to P56) shows that excitatory cell-type diversity emerges through continuous branching of progenitor lineages with branch points temporally controlled, rather than through abrupt fate switches — implying gradual transcriptomic specification of cortical glutamatergic cell types.

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  "doi": "10.1038/s41586-025-09644-1",
  "claim": "Single-cell and multi-omics atlas of 568,654 transcriptomes plus 200,061 nuclei sampled densely across mouse V1 development (E11.5 to P56) shows that excitatory cell-type diversity emerges through continuous branching of progenitor lineages with branch points temporally controlled, rather than through abrupt fate switches — implying gradual transcriptomic specification of cortical glutamatergic cell types.",
  "cite_key": "Gao2025",
  "evidence": "Single-cell RNA-seq (568,654 cells) and snMultiome (200,061 nuclei) of mouse visual cortex sampled E11.5 → P56, with computational trajectory analysis.",
  "effect_size": "quantitative — 568,654 cells + 200,061 nuclei across embryonic to adult V1; continuous lineage branching",
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  "study_system": "developing mouse primary visual cortex (V1) E11.5 to P56",
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  "claim_source_sentence": "We computationally reconstructed a transcriptomic developmental trajectory map of all excitatory, inhibitory and non-neuronal cell types in the visual cortex. Branching points that mark the emergence of new cell types at specific developmental ages and molecular signatures of cellular diversification are identified",
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  "effect_size_source_sentence": "568,654 high-quality single-cell transcriptomes and a single-nucleus Multiome dataset of 200,061 high-quality nuclei"
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We computationally reconstructed a transcriptomic developmental trajectory map of all excitatory, inhibitory and non-neuronal cell types in the visual cortex. Branching points that mark the emergence of new cell types at specific developmental ages and molecular signatures of cellular diversification are identified

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