A developmental transcriptomic and epigenomic atlas of mouse visual cortex buil…

A developmental transcriptomic and epigenomic atlas of mouse visual cortex built from 568,654 scRNA-seq cells and 200,061 multiome nuclei reveals continuous, staggered emergence of cortical cell types, including late emergence during the critical-period onset — demonstrating that single-snapshot Patch-seq taxonomies miss developmentally derived structure.

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  "claim": "A developmental transcriptomic and epigenomic atlas of mouse visual cortex built from 568,654 scRNA-seq cells and 200,061 multiome nuclei reveals continuous, staggered emergence of cortical cell types, including late emergence during the critical-period onset — demonstrating that single-snapshot Patch-seq taxonomies miss developmentally derived structure.",
  "cite_key": "Gao2025",
  "evidence": "Sets the present-day scale of mouse-cortex transcriptomic profiling and highlights what is missed without dense temporal sampling — relevant to cluster_14's outlook for large-scale Patch-seq.",
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The atlas is built from a single-cell RNA sequencing dataset of 568,654 high-quality single-cell transcriptomes and a single-nucleus Multiome dataset of 200,061 high-quality nuclei, which were densely sampled across the embryonic and postnatal developmental stages (from embryonic day 11.5 to postnatal day 56).

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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