MAPseq (Multiplexed Analysis of Projections by Sequencing) introduces high-thro…

MAPseq (Multiplexed Analysis of Projections by Sequencing) introduces high-throughput single-neuron axonal-projection mapping by labeling thousands of neurons with random RNA barcodes and sequencing barcode mRNA from target areas; applied to mouse locus coeruleus, it revealed that individual LC neurons have preferred (non-uniform) cortical targets, establishing the principle that bulk-tracing-homogeneous populations contain heterogeneous single-cell projection profiles.

{
  "n": 0,
  "doi": "10.1016/j.neuron.2016.07.036",
  "claim": "MAPseq (Multiplexed Analysis of Projections by Sequencing) introduces high-throughput single-neuron axonal-projection mapping by labeling thousands of neurons with random RNA barcodes and sequencing barcode mRNA from target areas; applied to mouse locus coeruleus, it revealed that individual LC neurons have preferred (non-uniform) cortical targets, establishing the principle that bulk-tracing-homogeneous populations contain heterogeneous single-cell projection profiles.",
  "cite_key": "Kebschull2016",
  "evidence": "Sindbis-virus-mediated random-barcode labeling of mouse locus coeruleus and target-area cDNA sequencing.",
  "effect_size": "qualitative — methodological introduction of high-throughput barcode-based single-neuron projection mapping",
  "text_access": "fulltext",
  "study_system": "mouse locus coeruleus and cortical targets (method validation)",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences ('barcodes'). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1038/nature26159",
    "10.1101/2025.06.25.661405"
  ],
  "effect_size_source_sentence": "individual LC neurons have preferred cortical targets"
}

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MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences ('barcodes'). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced

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