- claim_text
The MouseLight platform integrates a high-speed two-photon microscope with serial vibratome sectioning and large-scale image computation to enable whole-mouse-brain reconstruction of axonal arbors as thin as ~100 nm — establishing the methodological baseline for single-neuron projectome work in mouse motor cortex.
- raw_fields
{
"n": 0,
"doi": "10.7554/elife.10566",
"claim": "The MouseLight platform integrates a high-speed two-photon microscope with serial vibratome sectioning and large-scale image computation to enable whole-mouse-brain reconstruction of axonal arbors as thin as ~100 nm — establishing the methodological baseline for single-neuron projectome work in mouse motor cortex.",
"cite_key": "Economo2016",
"evidence": "Whole-mouse-brain 3D imaging at sub-diffraction axon resolution (100 nm); demonstrated reconstruction of multiple motor-cortex axonal arbors in one mouse.",
"effect_size": "reconstruction of axons ≥100 nm diameter spanning entire mouse brain",
"text_access": "fulltext",
"study_system": "Whole-mouse-brain TissueCyte+2P imaging (MouseLight)",
"argument_role": "supporting",
"replication_status": "platform-paper",
"claim_source_sentence": "We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data.",
"source_provenance_status": "ok",
"replication_evidence_dois": [],
"effect_size_source_sentence": "axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue."
}- source_refs
[
"paper:b99d7c4a-9270-42c0-b083-2109f9c1eabf"
]
- source_span
We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data.
- evidence_refs
[
{
"ref": "paper:b99d7c4a-9270-42c0-b083-2109f9c1eabf"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}