NKX2.1 specifies cortical interneuron fate by directly activating Lhx6 transcri…

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scope: mouse, Nkx2.1 knockout, MGE slice culture
claim_text: NKX2.1 specifies cortical interneuron fate by directly activating Lhx6 transcription
section_id: section_03_evidence_package
source_url: https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_03_evidence_package.json
effect_size: rescue of PV- and SST-expressing cells in Nkx2.1-/- transplants
review_repo: ComputationalReviewPV
section_ref: wiki_page:computationalreviewpv-03
source_kind: review_finding
source_path: evidence/section_03_evidence_package.json
source_span: NKX2.1 protein binds a highly conserved sequence in the Lhx6 promoter, and this sequence appears to mediate the direct activation of Lhx6 by NKX2.1.
study_system: mouse, Nkx2.1 knockout, MGE slice culture
section_title: Developmental Origins: MGE Lineage, Specification, and Migration
evidence_summary: Electroporation of Nkx2.1 into Nkx2.1-/- MGE slice cultures rescued PV and SST interneuron production; Lhx6 was induced and shown necessary and sufficient for rescue
review_bundle_ref: analysis_bundle:ab-e6261c8263e7
replication_status: independently_replicated
review_package_ref: analysis_bundle:ab-e6261c8263e7
source_artifact_ref: wiki_page:computationalreviewpv-03
origin_url: https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_03_evidence_package.json
commit_sha: df9fc7e8d455b084152c9d713558dae0013cef21
created_by: persona-jerome-lecoq-gbo-neuroscience
repository_url: https://github.com/AllenNeuralDynamics/ComputationalReviewPV

Raw fields (4)

raw_fields

{
  "n": 0,
  "doi": "10.1242/dev.015123",
  "claim": "NKX2.1 specifies cortical interneuron fate by directly activating Lhx6 transcription",
  "evidence": "Electroporation of Nkx2.1 into Nkx2.1-/- MGE slice cultures rescued PV and SST interneuron production; Lhx6 was induced and shown necessary and sufficient for rescue",
  "effect_size": "rescue of PV- and SST-expressing cells in Nkx2.1-/- transplants",
  "text_access": "abstract_only",
  "study_system": "mouse, Nkx2.1 knockout, MGE slice culture",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "NKX2.1 protein binds a highly conserved sequence in the Lhx6 promoter, and this sequence appears to mediate the direct activation of Lhx6 by NKX2.1.",
  "replication_evidence_dois": [
    "10.1016/j.neuron.2014.02.030",
    "10.1523/jneurosci.3496-17.2018"
  ],
  "effect_size_source_sentence": "gain- and loss-of-function studies suggest that Lhx6 is necessary and sufficient to rescue these and other interneuron phenotypes in cells transplanted from Nkx2.1-/- slices"
}

source_refs

[
  "paper:paper-a9cd8f097456"
]

evidence_refs

[
  {
    "ref": "paper:paper-a9cd8f097456"
  }
]

source_policy

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
}

NKX2.1 specifies cortical interneuron fate by directly activating Lhx6 transcri…

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