- claim_text
A substantial proportion of layer 5 cortical neurons labeled by the standard PV-Cre/tdTomato line are excitatory rather than inhibitory; the Vgat-Cre/PV-Flp/tdTomato intersectional combination provides specific labeling of GABAergic PV interneurons.
- raw_fields
{
"n": 7,
"doi": "10.1038/s41598-024-52901-y",
"claim": "A substantial proportion of layer 5 cortical neurons labeled by the standard PV-Cre/tdTomato line are excitatory rather than inhibitory; the Vgat-Cre/PV-Flp/tdTomato intersectional combination provides specific labeling of GABAergic PV interneurons.",
"cite_key": "Palicz2024",
"evidence": "Quantitative colocalization with GAD1, Vglut1, WFA in single Cre vs. intersectional reporter lines.",
"effect_size": "5 PV",
"text_access": "fulltext",
"study_system": "mouse cortex layer 5",
"source_cluster_id": "cluster_10",
"replication_status": "contested",
"claim_source_sentence": "The colocalization of the transgene (tdTomato) with the parvalbumin protein, with GAD1 (a conclusive inhibitory cell marker) and Vglut1 (a conclusive excitatory cell marker) as well as with a marker for perineuronal nets (WFA) was assessed and a substantial proportion of layer 5 PV neurons was found to be excitatory and not inhibitory in the PV-Cre/tdTomato mouse.",
"replication_evidence_dois": [],
"effect_size_source_sentence": "The colocalization of the transgene (tdTomato) with the parvalbumin protein, with GAD1 (a conclusive inhibitory cell marker) and Vglut1 (a conclusive excitatory cell marker) as well as with a marker for perineuronal nets (WFA) was assessed and a substantial proportion of layer 5 PV neurons was found to be excitatory and not inhibitory in the PV-Cre/tdTomato mouse."
}- source_refs
[
"paper:paper-c4df4e8ee950"
]
- source_span
The colocalization of the transgene (tdTomato) with the parvalbumin protein, with GAD1 (a conclusive inhibitory cell marker) and Vglut1 (a conclusive excitatory cell marker) as well as with a marker for perineuronal nets (WFA) was assessed and a substantial proportion of layer 5 PV neurons was found to be excitatory and not inhibitory in the PV-Cre/tdTomato mouse.
- evidence_refs
[
{
"ref": "paper:paper-c4df4e8ee950"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
}