Details
- scope
- C57BL/6 mouse, CRISPR-Cas9 knock-in line
- claim_text
- A new Drd1-P2A-Flpo knock-in mouse line drives Flp recombinase in D1 dopamine receptor-expressing neurons brain-wide and is suitable for intersectional genetic dissection.
- section_id
- section_11
- source_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json
- review_repo
- ComputationalReviewLoops
- section_ref
- wiki_page:computationalreviewloops-11
- source_kind
- review_finding
- source_path
- evidence/section_11_evidence_package.json
- source_span
- New method To address this limitation, we developed a novel Drd1-P2A-Flpo (Drd1-Flpo) mouse line in which the Flpo gene was knocked in immediately after the Drd1 gene using CRISPR-Cas9.
- study_system
- C57BL/6 mouse, CRISPR-Cas9 knock-in line
- section_title
- Cell-Type-Specific Dissection of Loop Circuits (Opto/Chemo/Tracing)
- evidence_summary
- Validated by Flp expression in D1DR+ neurons and brain-wide GFP reporter; intended for combinatorial use with existing Cre lines.
- review_bundle_ref
- analysis_bundle:ab-d49e54403ef9
- replication_status
- replication_unknown
- review_package_ref
- analysis_bundle:ab-d49e54403ef9
- source_artifact_ref
- wiki_page:computationalreviewloops-11
- origin_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json
- commit_sha
- 0632aae8abc141909207fe91f6349b9e36489c3b
- created_by
- persona-jerome-lecoq-gbo-neuroscience
- repository_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewLoops
Raw fields (4)
- raw_fields
{ "n": 0, "doi": "10.1101/2024.04.25.591164", "claim": "A new Drd1-P2A-Flpo knock-in mouse line drives Flp recombinase in D1 dopamine receptor-expressing neurons brain-wide and is suitable for intersectional genetic dissection.", "cite_key": "Oppman2024", "evidence": "Validated by Flp expression in D1DR+ neurons and brain-wide GFP reporter; intended for combinatorial use with existing Cre lines.", "effect_size": null, "text_access": "abstract_only", "study_system": "C57BL/6 mouse, CRISPR-Cas9 knock-in line", "source_cluster_id": "cluster_10", "replication_status": "replication_unknown", "claim_source_sentence": "New method To address this limitation, we developed a novel Drd1-P2A-Flpo (Drd1-Flpo) mouse line in which the Flpo gene was knocked in immediately after the Drd1 gene using CRISPR-Cas9.", "replication_evidence_dois": [], "effect_size_source_sentence": null }- source_refs
[ "paper:paper-c26676d2b6bb" ]
- evidence_refs
[ { "ref": "paper:paper-c26676d2b6bb" } ]- source_policy
{ "mode": "public_source_pointer_with_short_context", "notes": [ "Local review repositories are read-only inputs.", "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose." ], "source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b", "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops" }