A multi-unit microscope performing simultaneous two-photon imaging and two-phot…

A multi-unit microscope performing simultaneous two-photon imaging and two-photon multiplexed holographic optogenetics at axially distinct planes mapped the functional connectivity between rod bipolar cells and ganglion cells in mouse retina with cell-type specificity and single-cell resolution — establishing the all-optical inter-layer-circuit interrogation methodology with direct translation to mouse-cortex laminar work.

{
  "n": 0,
  "doi": "10.1016/j.crmeth.2022.100268",
  "claim": "A multi-unit microscope performing simultaneous two-photon imaging and two-photon multiplexed holographic optogenetics at axially distinct planes mapped the functional connectivity between rod bipolar cells and ganglion cells in mouse retina with cell-type specificity and single-cell resolution — establishing the all-optical inter-layer-circuit interrogation methodology with direct translation to mouse-cortex laminar work.",
  "cite_key": "Spampinato2022",
  "evidence": "Inter-layer functional connectivity mapping with cell-type-specific holographic stim.",
  "effect_size": "cell-type-specific inter-layer functional connectivity mapped (RBC→GC) at single-cell resolution",
  "text_access": "fulltext",
  "study_system": "Mouse retina (method translates to cortex)",
  "argument_role": "supporting",
  "replication_status": "instrument-paper",
  "claim_source_sentence": "We developed a multi-unit microscope for all-optical inter-layers circuits interrogation. The system performs two-photon (2P) functional imaging and 2P multiplexed holographic optogenetics at axially distinct planes.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "We demonstrated the capability of the system to map, in the mouse retina, the functional connectivity between rod bipolar cells (RBCs) and ganglion cells (GCs) by activating single or defined groups of RBCs while recording the evoked response in the GC layer with cell-type specificity and single-cell resolution."
}

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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