Multiplexed miniaturized two-photon microscopes can record calcium activity fro…

Multiplexed miniaturized two-photon microscopes can record calcium activity from freely moving mice across a 500×500 µm FOV at increased frame rate and enable 2P voltage imaging at 400 Hz over a 380×150 µm FOV — bounding the present envelope of freely-moving 2P recording.

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  "n": 0,
  "doi": "10.1016/j.crmeth.2025.101221",
  "claim": "Multiplexed miniaturized two-photon microscopes can record calcium activity from freely moving mice across a 500×500 µm FOV at increased frame rate and enable 2P voltage imaging at 400 Hz over a 380×150 µm FOV — bounding the present envelope of freely-moving 2P recording.",
  "cite_key": "Zhang2025b",
  "evidence": "Quantitative bound on what miniature 2P delivers today in freely behaving mice — relevant to cluster_14's tool-limit discussion.",
  "effect_size": "500 × 500 µm² calcium FOV; 400 Hz voltage frame rate over 380 × 150 µm²",
  "text_access": "abstract_only",
  "study_system": "Freely moving mouse visual and prefrontal cortex; M-MINI2P head-mounted 2P microscope",
  "argument_role": "supporting",
  "replication_status": "single-study",
  "claim_source_sentence": "The increased speed of M-MINI2Ps also enables two-photon voltage imaging at 400 Hz over a 380 × 150 μm<sup>2</sup> FOV in freely moving mice.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "We demonstrate large-scale (500 × 500 μm<sup>2</sup> FOV) multiplane calcium imaging in visual and prefrontal cortices of freely moving mice during spontaneous exploration, social behavior, and auditory stimulus."
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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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